Cadmium in PDB 1et7: Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6

All present enzymatic activity of Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6:
1.7.99.3;

The structure of Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6, PDB code: 1et7 was solved by M.J.Boulanger, M.Kukimoto, M.Nishiyama, S.Horinouchi, M.E.P.Murphy, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	500.00 / 1.70
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	127.640, 127.640, 67.510, 90.00, 90.00, 120.00
R / Rfree (%)	18.8 / 21

The structure of Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6 also contains other interesting chemical elements:

Copper

(Cu)

2 atoms

The binding sites of Cadmium atom in the Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6 (pdb code 1et7). This binding sites where shown within 5.0 Angstroms radius around Cadmium atom.
In total 3 binding sites of Cadmium where determined in the Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6, PDB code: 1et7:
Jump to Cadmium binding site number: 1; 2; 3;

Cadmium binding site 1 out of 3 in the Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 1 of Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cd504 b:24.4 occ:0.33 O A:HOH662 2.2 26.6 1.0 O A:HOH627 2.4 24.9 1.0 O A:HOH796 3.9 40.5 1.0 O A:HOH784 4.3 40.6 1.0 OD1 A:ASP275 4.4 23.6 1.0 OD2 A:ASP275 4.4 22.5 1.0 CG A:ASP275 4.8 22.5 1.0

Cadmium binding site 2 out of 3 in the Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 2 of Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cd505 b:28.7 occ:1.00 O A:HOH730 1.6 25.4 1.0 OE2 A:GLU89 2.3 37.3 1.0 OE1 A:GLU89 2.8 37.5 1.0 CD A:GLU89 2.9 33.9 1.0 O A:HOH754 4.0 47.8 1.0 CG A:GLU89 4.4 31.2 1.0

Cadmium binding site 3 out of 3 in the Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 3 of Crystal Structure of Nitrite Reductase HIS255ASP Mutant From Alcaligenes Faecalis S-6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cd506 b:44.7 occ:0.30 OE1 A:GLU8 2.4 44.1 1.0 OE2 A:GLU8 2.5 43.3 1.0 CD A:GLU8 2.7 41.8 1.0 CG A:GLU8 4.2 38.5 1.0 CG2 A:THR5 4.4 34.7 1.0 N A:THR5 4.6 35.5 1.0 CA A:ALA4 4.6 37.2 1.0 O A:HOH691 5.0 53.7 1.0

M.J.Boulanger, M.Kukimoto, M.Nishiyama, S.Horinouchi, M.E.Murphy. Catalytic Roles For Two Water Bridged Residues (Asp-98 and His-255) in the Active Site of Copper-Containing Nitrite Reductase. J.Biol.Chem. V. 275 23957 2000.
ISSN: ISSN 0021-9258
PubMed: 10811642
DOI: 10.1074/JBC.M001859200