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Cadmium in PDB 1gl0: Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria

Enzymatic activity of Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria

All present enzymatic activity of Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria:
3.4.21.1;

Protein crystallography data

The structure of Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria, PDB code: 1gl0 was solved by A.Roussel, C.Kellenberger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 14.98 / 3.0
Space group P 65 2 2
Cell size a, b, c (Å), α, β, γ (°) 85.853, 85.853, 187.983, 90.00, 90.00, 120.00
R / Rfree (%) 17.4 / 19.8

Cadmium Binding Sites:

The binding sites of Cadmium atom in the Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria (pdb code 1gl0). This binding sites where shown within 5.0 Angstroms radius around Cadmium atom.
In total 3 binding sites of Cadmium where determined in the Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria, PDB code: 1gl0:
Jump to Cadmium binding site number: 1; 2; 3;

Cadmium binding site 1 out of 3 in 1gl0

Go back to Cadmium Binding Sites List in 1gl0
Cadmium binding site 1 out of 3 in the Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 1 of Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Cd1246

b:19.0
occ:1.00
OD2 E:ASP153 2.0 29.5 1.0
O E:HOH2014 2.3 11.2 1.0
OD1 E:ASP72 2.5 15.5 1.0
OD2 E:ASP72 2.5 17.4 1.0
CG E:ASP72 2.9 15.8 1.0
CG E:ASP153 3.1 31.6 1.0
CB E:ASP153 3.7 30.2 1.0
OD1 E:ASP153 4.1 33.6 1.0
CA E:GLY74 4.3 23.0 1.0
CB E:ASP72 4.4 15.3 1.0
N E:GLY74 4.5 22.0 1.0
C E:GLY74 4.5 23.8 1.0
N E:SER75 4.5 23.9 1.0
NE E:ARG154 4.8 31.0 1.0

Cadmium binding site 2 out of 3 in 1gl0

Go back to Cadmium Binding Sites List in 1gl0
Cadmium binding site 2 out of 3 in the Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 2 of Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Cd1247

b:24.7
occ:1.00
OE1 E:GLU49 2.2 14.1 1.0
OE2 E:GLU49 2.6 15.6 1.0
CD E:GLU49 2.7 13.5 1.0
CG E:GLU49 4.2 11.4 1.0
CE2 E:PHE114 4.3 20.1 1.0
CD2 E:PHE114 4.8 20.6 1.0
N E:GLU49 4.8 12.6 1.0

Cadmium binding site 3 out of 3 in 1gl0

Go back to Cadmium Binding Sites List in 1gl0
Cadmium binding site 3 out of 3 in the Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 3 of Structure of the Complex Between Bovine Alpha-Chymotrypsin and Pmp-D2V, An Inhibitor From the Insect Locusta Migratoria within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Cd1248

b:77.8
occ:1.00
O E:HOH2008 2.5 23.9 1.0
OXT E:ASN245 2.7 26.2 1.0
O E:ASN245 3.1 25.3 1.0
C E:ASN245 3.2 25.9 1.0
NZ E:LYS107 4.2 24.7 1.0
O E:LEU242 4.3 16.3 1.0
CE E:LYS107 4.6 22.8 1.0
NE1 E:TRP51 4.6 19.5 1.0
CA E:ASN245 4.7 25.7 1.0
CZ2 E:TRP51 4.9 19.9 1.0

Reference:

A.Roussel, M.Mathieu, A.Dobbs, B.Luu, C.Cambillau, C.Kellenberger. Complexation of Two Proteic Insect Inhibitors to the Active Site of Chymotrypsin Suggests Decoupled Roles For Binding and Selectivity J.Biol.Chem. V. 276 38893 2001.
ISSN: ISSN 0021-9258
PubMed: 11495915
DOI: 10.1074/JBC.M105707200
Page generated: Fri Jul 19 13:26:18 2024

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