Cadmium in PDB 1yj9: Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22
Protein crystallography data
The structure of Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22, PDB code: 1yj9
was solved by
D.Tu,
G.Blaha,
P.B.Moore,
T.A.Steitz,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
29.94 /
2.90
|
Space group
|
C 2 2 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
213.076,
300.750,
575.478,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
18.4 /
24.2
|
Other elements in 1yj9:
The structure of Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22 also contains other interesting chemical elements:
Cadmium Binding Sites:
The binding sites of Cadmium atom in the Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22
(pdb code 1yj9). This binding sites where shown within
5.0 Angstroms radius around Cadmium atom.
In total 5 binding sites of Cadmium where determined in the
Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22, PDB code: 1yj9:
Jump to Cadmium binding site number:
1;
2;
3;
4;
5;
Cadmium binding site 1 out
of 5 in 1yj9
Go back to
Cadmium Binding Sites List in 1yj9
Cadmium binding site 1 out
of 5 in the Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22
Mono view
Stereo pair view
|
A full contact list of Cadmium with other atoms in the Cd binding
site number 1 of Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
O:Cd8705
b:98.1
occ:1.00
|
ND1
|
O:HIS40
|
2.6
|
44.3
|
1.0
|
O
|
O:HOH5650
|
2.9
|
44.6
|
1.0
|
O
|
0:HOH5824
|
3.2
|
25.0
|
1.0
|
CE1
|
O:HIS40
|
3.5
|
43.5
|
1.0
|
CG
|
O:HIS40
|
3.6
|
44.3
|
1.0
|
CB
|
O:HIS40
|
3.9
|
46.6
|
1.0
|
CG
|
O:ARG37
|
4.3
|
59.7
|
1.0
|
NH1
|
O:ARG37
|
4.5
|
61.8
|
1.0
|
CZ
|
O:ARG37
|
4.5
|
62.4
|
1.0
|
NE2
|
O:HIS40
|
4.6
|
43.5
|
1.0
|
CB
|
O:ARG37
|
4.7
|
57.1
|
1.0
|
CD2
|
O:HIS40
|
4.7
|
43.3
|
1.0
|
NE
|
O:ARG37
|
4.7
|
62.6
|
1.0
|
O
|
O:HOH3002
|
4.8
|
57.7
|
1.0
|
CA
|
O:ARG37
|
4.8
|
56.2
|
1.0
|
NH2
|
O:ARG37
|
4.9
|
60.4
|
1.0
|
O
|
O:ARG37
|
4.9
|
56.8
|
1.0
|
O
|
0:HOH9906
|
5.0
|
45.3
|
1.0
|
|
Cadmium binding site 2 out
of 5 in 1yj9
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Cadmium Binding Sites List in 1yj9
Cadmium binding site 2 out
of 5 in the Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22
Mono view
Stereo pair view
|
A full contact list of Cadmium with other atoms in the Cd binding
site number 2 of Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
U:Cd8701
b:76.4
occ:1.00
|
SG
|
U:CYS36
|
2.3
|
68.7
|
1.0
|
SG
|
U:CYS32
|
2.3
|
45.8
|
1.0
|
SG
|
U:CYS9
|
2.5
|
66.1
|
1.0
|
SG
|
U:CYS6
|
2.5
|
55.5
|
1.0
|
CB
|
U:CYS6
|
3.2
|
59.0
|
1.0
|
CB
|
U:CYS9
|
3.6
|
63.1
|
1.0
|
CB
|
U:CYS32
|
3.6
|
52.9
|
1.0
|
CB
|
U:CYS36
|
3.7
|
65.2
|
1.0
|
N
|
U:CYS32
|
4.0
|
54.1
|
1.0
|
N
|
U:CYS9
|
4.0
|
61.9
|
1.0
|
CA
|
U:CYS32
|
4.3
|
55.3
|
1.0
|
OG1
|
U:THR11
|
4.3
|
70.7
|
1.0
|
OG
|
U:SER33
|
4.3
|
60.4
|
1.0
|
CA
|
U:CYS9
|
4.4
|
62.8
|
1.0
|
N
|
U:SER33
|
4.5
|
59.8
|
1.0
|
CA
|
U:CYS6
|
4.7
|
60.0
|
1.0
|
CB
|
U:TYR8
|
4.7
|
58.2
|
1.0
|
C
|
U:CYS32
|
4.8
|
58.0
|
1.0
|
N
|
U:GLY10
|
4.8
|
63.9
|
1.0
|
CA
|
U:CYS36
|
5.0
|
63.2
|
1.0
|
|
Cadmium binding site 3 out
of 5 in 1yj9
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Cadmium Binding Sites List in 1yj9
Cadmium binding site 3 out
of 5 in the Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22
Mono view
Stereo pair view
|
A full contact list of Cadmium with other atoms in the Cd binding
site number 3 of Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
Z:Cd8703
b:71.1
occ:1.00
|
SG
|
Z:CYS39
|
2.3
|
61.4
|
1.0
|
SG
|
Z:CYS60
|
2.3
|
66.1
|
1.0
|
SG
|
Z:CYS57
|
2.4
|
72.4
|
1.0
|
SG
|
Z:CYS42
|
2.6
|
77.6
|
1.0
|
CB
|
Z:CYS60
|
3.1
|
68.2
|
1.0
|
CB
|
Z:CYS42
|
3.2
|
78.8
|
1.0
|
CB
|
Z:CYS57
|
3.2
|
73.8
|
1.0
|
CB
|
Z:CYS39
|
3.2
|
67.0
|
1.0
|
N
|
Z:CYS42
|
3.3
|
80.3
|
1.0
|
N
|
Z:CYS60
|
3.7
|
71.8
|
1.0
|
CB
|
Z:ASN41
|
3.8
|
80.2
|
1.0
|
CA
|
Z:CYS42
|
3.8
|
79.5
|
1.0
|
CA
|
Z:CYS60
|
3.9
|
69.4
|
1.0
|
C
|
Z:ASN41
|
4.3
|
80.2
|
1.0
|
CD2
|
Z:TYR59
|
4.5
|
84.3
|
1.0
|
CA
|
Z:ASN41
|
4.5
|
79.5
|
1.0
|
CB
|
Z:TYR62
|
4.6
|
63.7
|
1.0
|
CB
|
Z:TYR59
|
4.6
|
80.0
|
1.0
|
N
|
Z:ASN41
|
4.6
|
78.7
|
1.0
|
N
|
Z:GLY43
|
4.7
|
78.7
|
1.0
|
CA
|
Z:CYS39
|
4.7
|
69.2
|
1.0
|
C
|
Z:CYS60
|
4.7
|
70.2
|
1.0
|
CA
|
Z:CYS57
|
4.7
|
73.6
|
1.0
|
C
|
Z:CYS42
|
4.8
|
79.2
|
1.0
|
C
|
Z:TYR59
|
4.9
|
75.1
|
1.0
|
N
|
Z:ASP61
|
4.9
|
70.0
|
1.0
|
N
|
Z:TYR62
|
4.9
|
66.8
|
1.0
|
CG
|
Z:ASN41
|
5.0
|
80.4
|
1.0
|
|
Cadmium binding site 4 out
of 5 in 1yj9
Go back to
Cadmium Binding Sites List in 1yj9
Cadmium binding site 4 out
of 5 in the Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22
Mono view
Stereo pair view
|
A full contact list of Cadmium with other atoms in the Cd binding
site number 4 of Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
1:Cd8702
b:68.8
occ:1.00
|
SG
|
1:CYS37
|
2.3
|
45.2
|
1.0
|
SG
|
1:CYS22
|
2.4
|
44.6
|
1.0
|
SG
|
1:CYS34
|
2.5
|
45.5
|
1.0
|
SG
|
1:CYS19
|
2.5
|
47.0
|
1.0
|
CB
|
1:CYS19
|
3.1
|
42.8
|
1.0
|
CB
|
1:CYS22
|
3.2
|
41.0
|
1.0
|
CB
|
1:CYS37
|
3.2
|
39.5
|
1.0
|
CB
|
1:CYS34
|
3.2
|
39.7
|
1.0
|
N
|
1:CYS22
|
3.6
|
42.2
|
1.0
|
N
|
1:CYS37
|
3.8
|
38.9
|
1.0
|
CA
|
1:CYS22
|
3.9
|
41.9
|
1.0
|
CA
|
1:CYS37
|
4.1
|
38.6
|
1.0
|
CB
|
1:PHE39
|
4.3
|
45.9
|
1.0
|
CB
|
1:ARG21
|
4.5
|
43.3
|
1.0
|
C
|
1:ARG21
|
4.6
|
43.3
|
1.0
|
CA
|
1:CYS19
|
4.6
|
44.1
|
1.0
|
C
|
1:CYS22
|
4.6
|
41.7
|
1.0
|
CA
|
1:CYS34
|
4.7
|
39.2
|
1.0
|
O
|
1:HOH1020
|
4.7
|
41.3
|
1.0
|
C
|
1:CYS37
|
4.8
|
38.1
|
1.0
|
CB
|
1:SER36
|
4.8
|
38.0
|
1.0
|
N
|
1:GLY23
|
4.8
|
41.9
|
1.0
|
CD
|
1:ARG21
|
4.8
|
44.2
|
1.0
|
N
|
1:PHE39
|
4.9
|
43.4
|
1.0
|
CA
|
1:ARG21
|
4.9
|
43.1
|
1.0
|
N
|
1:GLY38
|
4.9
|
39.3
|
1.0
|
C
|
1:SER36
|
4.9
|
39.2
|
1.0
|
N
|
1:ARG21
|
4.9
|
44.0
|
1.0
|
|
Cadmium binding site 5 out
of 5 in 1yj9
Go back to
Cadmium Binding Sites List in 1yj9
Cadmium binding site 5 out
of 5 in the Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22
Mono view
Stereo pair view
|
A full contact list of Cadmium with other atoms in the Cd binding
site number 5 of Crystal Structure of the Mutant 50S Ribosomal Subunit of Haloarcula Marismortui Containing A Three Residue Deletion in L22 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
3:Cd8704
b:72.6
occ:1.00
|
SG
|
3:CYS14
|
2.2
|
77.0
|
1.0
|
SG
|
3:CYS71
|
2.3
|
54.0
|
1.0
|
SG
|
3:CYS11
|
2.3
|
70.8
|
1.0
|
SG
|
3:CYS74
|
2.3
|
70.5
|
1.0
|
CB
|
3:CYS71
|
2.9
|
59.1
|
1.0
|
CB
|
3:CYS11
|
3.5
|
66.5
|
1.0
|
O
|
3:HOH8858
|
3.5
|
0.6
|
1.0
|
CB
|
3:CYS74
|
3.5
|
70.2
|
1.0
|
CB
|
3:CYS14
|
3.6
|
73.2
|
1.0
|
N
|
3:CYS74
|
4.0
|
71.0
|
1.0
|
N
|
3:CYS14
|
4.1
|
72.0
|
1.0
|
CA
|
3:CYS74
|
4.3
|
70.9
|
1.0
|
CA
|
3:CYS71
|
4.4
|
60.5
|
1.0
|
CA
|
3:CYS14
|
4.5
|
72.9
|
1.0
|
CB
|
3:HIS13
|
4.6
|
70.9
|
1.0
|
O
|
3:HOH8836
|
4.7
|
54.6
|
1.0
|
N
|
3:GLY75
|
4.8
|
70.3
|
1.0
|
C
|
3:CYS71
|
4.8
|
62.0
|
1.0
|
C
|
3:CYS74
|
4.9
|
70.6
|
1.0
|
CA
|
3:CYS11
|
4.9
|
65.8
|
1.0
|
O
|
3:CYS71
|
4.9
|
62.4
|
1.0
|
O
|
3:LYS76
|
5.0
|
71.4
|
1.0
|
|
Reference:
D.Tu,
G.Blaha,
P.B.Moore,
T.A.Steitz.
Structures of Mlsbk Antibiotics Bound to Mutated Large Ribosomal Subunits Provide A Structural Explanation For Resistance. Cell(Cambridge,Mass.) V. 121 257 2005.
ISSN: ISSN 0092-8674
PubMed: 15851032
DOI: 10.1016/J.CELL.2005.02.005
Page generated: Fri Jul 19 14:35:14 2024
|