Cadmium in PDB 3cn6: Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant

The structure of Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant, PDB code: 3cn6 was solved by M.Nyblom, A.Alfredsson, K.Hallgren, K.Hedfalk, R.Neutze, S.Tornroth-Horsefield, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.86 / 2.95
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	89.558, 89.558, 189.271, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 23

The binding sites of Cadmium atom in the Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant (pdb code 3cn6). This binding sites where shown within 5.0 Angstroms radius around Cadmium atom.
In total 2 binding sites of Cadmium where determined in the Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant, PDB code: 3cn6:
Jump to Cadmium binding site number: 1; 2;

Cadmium binding site 1 out of 2 in the Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 1 of Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cd282 b:32.3 occ:1.00 OD1 A:ASP28 1.9 42.0 1.0 OE2 A:GLU31 2.0 35.0 1.0 OD2 A:ASP28 2.2 42.3 1.0 CG A:ASP28 2.3 38.0 1.0 O A:HOH286 2.4 15.6 1.0 O A:HOH288 2.5 18.7 1.0 CD A:GLU31 2.6 34.8 1.0 OE1 A:GLU31 2.8 33.3 1.0 CB A:ASP28 3.9 36.8 1.0 CG A:GLU31 4.1 34.6 1.0 OG A:SER115 4.3 26.9 1.0 NE A:ARG118 4.3 25.8 1.0 N A:GLU31 4.4 32.4 1.0 CA A:GLY30 4.5 29.8 1.0 CB A:SER115 4.5 25.7 1.0 C A:GLY30 4.5 31.2 1.0 N A:ASP28 4.5 38.3 1.0 N A:GLY30 4.5 30.2 1.0 NH2 A:ARG118 4.6 26.1 1.0 CA A:ASP28 4.7 36.2 1.0 O A:ASP28 4.8 34.5 1.0 C A:ASP28 4.9 34.5 1.0 CZ A:ARG118 4.9 22.4 1.0 N A:SER115 5.0 25.8 1.0

Cadmium binding site 2 out of 2 in the Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 2 of Crystal Structure of the Spinach Aquaporin SOPIP2;1 S274E Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cd282 b:31.6 occ:1.00 OE2 B:GLU31 2.1 37.2 1.0 OD1 B:ASP28 2.2 29.9 1.0 O B:HOH283 2.3 14.8 1.0 OD2 B:ASP28 2.4 35.6 1.0 O B:HOH284 2.5 20.1 1.0 CG B:ASP28 2.6 31.5 1.0 CD B:GLU31 2.9 37.5 1.0 OE1 B:GLU31 3.0 37.5 1.0 NE B:ARG118 4.1 19.2 1.0 CB B:ASP28 4.2 32.5 1.0 NH2 B:ARG118 4.3 21.6 1.0 CG B:GLU31 4.4 32.9 1.0 OG B:SER115 4.4 26.3 1.0 CB B:SER115 4.6 26.8 1.0 N B:GLU31 4.6 29.7 1.0 CA B:GLY30 4.7 27.9 1.0 CZ B:ARG118 4.7 18.9 1.0 N B:SER115 4.8 27.3 1.0 N B:GLY30 4.8 27.3 1.0 N B:ASP28 4.8 35.7 1.0 C B:GLY30 4.8 28.4 1.0 CA B:ASP28 4.9 32.8 1.0

M.Nyblom, A.Frick, Y.Wang, M.Ekvall, K.Hallgren, K.Hedfalk, R.Neutze, E.Tajkhorshid, S.Tornroth-Horsefield. Structural and Functional Analysis of SOPIP2;1 Mutants Adds Insight Into Plant Aquaporin Gating. J.Mol.Biol. V. 387 653 2009.
ISSN: ISSN 0022-2836
PubMed: 19302796
DOI: 10.1016/J.JMB.2009.01.065