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Cadmium in PDB 3eig: Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase

Enzymatic activity of Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase

All present enzymatic activity of Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase:
1.5.1.3;

Protein crystallography data

The structure of Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase, PDB code: 3eig was solved by B.J.Yachnin, A.M.Berghuis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 10.52 / 1.70
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 42.348, 47.868, 90.715, 90.00, 90.00, 90.00
R / Rfree (%) 17.4 / 22.3

Cadmium Binding Sites:

The binding sites of Cadmium atom in the Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase (pdb code 3eig). This binding sites where shown within 5.0 Angstroms radius around Cadmium atom.
In total only one binding site of Cadmium was determined in the Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase, PDB code: 3eig:

Cadmium binding site 1 out of 1 in 3eig

Go back to Cadmium Binding Sites List in 3eig
Cadmium binding site 1 out of 1 in the Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 1 of Crystal Structure of A Methotrexate-Resistant Mutant of Human Dihydrofolate Reductase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cd209

b:13.7
occ:0.75
 O4 A:SO4203 2.3 27.6 1.0
OE2 A:GLU183 2.5 16.4 1.0
OE1 A:GLU183 2.5 17.4 1.0
O A:HOH292 2.7 20.8 1.0
CD A:GLU183 2.8 15.8 1.0
S A:SO4203 3.2 25.4 1.0
O1 A:SO4203 3.4 24.9 1.0
O3 A:SO4203 3.5 25.8 1.0
CE A:LYS132 4.2 15.7 1.0
CG A:GLU183 4.3 15.5 1.0
NZ A:LYS132 4.3 17.4 1.0
CE1 A:HIS130 4.4 20.4 1.0
NE2 A:HIS130 4.4 21.1 1.0
O2 A:SO4203 4.5 24.9 1.0
O A:HOH253 4.9 27.9 1.0
CD1 A:LEU159 4.9 14.7 1.0

Reference:

J.P.Volpato, B.J.Yachnin, J.Blanchet, V.Guerrero, L.Poulin, E.Fossati, A.M.Berghuis, J.N.Pelletier. Multiple Conformers in Active Site of Human Dihydrofolate Reductase F31R/Q35E Double Mutant Suggest Structural Basis For Methotrexate Resistance. J.Biol.Chem. V. 284 20079 2009.
ISSN: ISSN 0021-9258
PubMed: 19478082
DOI: 10.1074/JBC.M109.018010
Page generated: Fri Aug 28 13:34:15 2020
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