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Cadmium in PDB 6ci0: Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation

Enzymatic activity of Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation

All present enzymatic activity of Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation:
1.9.3.1;

Protein crystallography data

The structure of Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation, PDB code: 6ci0 was solved by J.Liu, C.Hiser, S.Ferguson-Miller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 42.09 / 2.40
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 124.916, 130.969, 177.788, 90.00, 90.00, 90.00
R / Rfree (%) 19.9 / 23.4

Other elements in 6ci0:

The structure of Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Potassium (K) 1 atom
Iron (Fe) 4 atoms
Calcium (Ca) 2 atoms
Copper (Cu) 6 atoms

Cadmium Binding Sites:

The binding sites of Cadmium atom in the Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation (pdb code 6ci0). This binding sites where shown within 5.0 Angstroms radius around Cadmium atom.
In total 2 binding sites of Cadmium where determined in the Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation, PDB code: 6ci0:
Jump to Cadmium binding site number: 1; 2;

Cadmium binding site 1 out of 2 in 6ci0

Go back to Cadmium Binding Sites List in 6ci0
Cadmium binding site 1 out of 2 in the Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 1 of Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cd305

b:63.6
occ:1.00
OE1 B:GLU280 2.6 68.5 1.0
OE2 B:GLU280 2.6 65.0 1.0
NE2 B:HIS285 2.8 68.3 1.0
CD2 B:HIS283 2.9 62.4 1.0
CD B:GLU280 3.0 67.9 1.0
CD2 B:HIS285 3.5 70.6 1.0
CG B:HIS283 3.5 61.6 1.0
CB B:HIS283 3.6 51.8 1.0
CE1 B:HIS285 3.9 68.0 1.0
NE2 B:HIS283 4.1 71.6 1.0
CG B:GLU280 4.5 52.5 1.0
ND1 B:HIS283 4.7 80.9 1.0
CG B:HIS285 4.7 74.4 1.0
C4 B:HTH308 4.8 70.9 0.8
O B:GLU280 4.8 59.3 1.0
ND1 B:HIS285 4.9 79.0 1.0
CA B:HIS283 4.9 69.8 1.0
CE1 B:HIS283 5.0 80.1 1.0

Cadmium binding site 2 out of 2 in 6ci0

Go back to Cadmium Binding Sites List in 6ci0
Cadmium binding site 2 out of 2 in the Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation


Mono view


Stereo pair view

A full contact list of Cadmium with other atoms in the Cd binding site number 2 of Catalytic Core Subunits (I and II) of Cytochrome C Oxidase From Rhodobacter Sphaeroides with E101A (II) Mutation within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cd306

b:62.7
occ:1.00
OE1 D:GLU280 2.5 52.4 1.0
OE2 D:GLU280 2.6 54.4 1.0
ND1 D:HIS283 2.8 70.2 1.0
NE2 D:HIS285 2.8 69.2 1.0
CD D:GLU280 2.9 66.7 1.0
CG D:HIS283 3.6 77.8 1.0
CD2 D:HIS285 3.6 69.9 1.0
CB D:HIS283 3.6 63.9 1.0
CE1 D:HIS283 3.8 76.2 1.0
CE1 D:HIS285 3.9 63.6 1.0
CG D:GLU280 4.4 53.9 1.0
CD2 D:HIS283 4.7 83.8 1.0
NE2 D:HIS283 4.8 77.6 1.0
CG D:HIS285 4.8 74.2 1.0
O D:GLU280 4.9 59.3 1.0
ND1 D:HIS285 4.9 80.4 1.0

Reference:

C.Hiser, J.Liu, S.Ferguson-Miller. The K-Path Entrance in Cytochrome C Oxidase Is Defined By Mutation of E101 and Controlled By An Adjacent Ligand Binding Domain. Biochim. Biophys. Acta V.1859 725 2018.
ISSN: ISSN 0006-3002
PubMed: 29626419
DOI: 10.1016/J.BBABIO.2018.03.017
Page generated: Sat Dec 12 08:28:13 2020

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